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AssigneeQingdao Inst Bioenergy & Bioprocess Tech Cas
InventorsShengjun LI, Ruibo HU, Hua XU, Shumin WANG, Guo HE
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The present invention discloses the application of Duckweed Pericarp Color1 (P1) protein homologues (P1-Ls) such as LtP1-L or LeP1-L in regulating the biosynthesis of orientin and/or isoorientin in duckweed or other plants. Specifically, P1-L was introduced into the duckweed genome and overexpressed. The transgenic duckweed generates higher content of orientin and/or isoorientin than wild-type duckweed. The present invention also discloses a method for biosynthesizing orientin and/or isoorientin: P1-L was introduced into the duckweed genome, P1-L overexpressing duckweed with higher content of orientin and/or isoorientin than wild-type plants; Cultivation of the transgenic duckweed and extraction of orientin and/or isoorientin. The present invention greatly reduces the production costs of orientin and isoorientin, and has broad application prospects and high economic value.
A method for producing a plant with modified flavonoid biosynthesis, the method comprising altering the expression, or activity, of a Duckweed Pericarp Color1 (P1) protein homologue in the plant.
Transgenic duckweed having a content of orientin and isoorientin of about 0.29% to 0.37%, or about 0.53% to 0.73% respectively, more preferably about 0.29% or 0.37%, or about 0.53% or 0.73% respectively.
A culture solution of transgenic duckweed having a content of orientin and isoorientin of about 16.83 mg/L to 22.38 mg/L and 24.89 mg/L to 30.54 mg/L respectively, more preferably about 16.83 mg or 22.38 mg/L, or about 24.89 mg/L or 30.54 mg/L respectively.
Dependencies are inferred from the claim text.