Functionally enhanced 10-23 dna enzyme with chemically optimized catalytic core

Abstract

Molecules, materials, and methods for gene silencing utilize a modified 10-23 DNAzyme that functions with unparalleled catalytic activity under physiological conditions. The enzyme was discovered through iterative cycles of design that were guided by structural information available on the folding topology and metal-ion binding sites of Dz 10-23. The new enzyme can achieve ~65 turnovers in 30 minutes, a feat only previously witnessed by the unmodified parent sequence under forcing conditions of elevated Mg2+ and pH, making it the fastest known RNA-cleaving DNAzyme under physiological conditions.

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