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AssigneeOregon State University
InventorsRyan A. Mehl, Richard B. Cooley, Nathan D. Alexander
Estimating the term…
Pyrrolysine-based aminoacyl-tRNA synthetases competent to recognize a fluorinated phenylalanine analog and aminoacylate a tRNA; pyrrolysine-based aminoacyl-tRNA synthetase/tRNA pairs comprising the pyrrolysine-based aminoacyl-tRNA synthetase and a tRNA competent for site-specific encoding of a fluorinated phenylalanine analog within a protein; methods of site-specific encoding of a fluorinated phenylalanine analog in a cell, comprising transfecting/transforming/transducing a cell with the pyrrolysine-based aminoacyl-tRNA synthetase and the tRNA competent for site-specific encoding; and kits for in cellulo production of a fluorinated phenylalanine-labeled protein using DNA encoding the pyrrolysine-based aminoacyl-tRNA synthetase and the tRNA competent for site-specific encoding of a fluorinated phenylalanine analog, and a fluorinated phenylalanine analog.
A pyrrolysine-based aminoacyl-tRNA synthetase competent to recognize a fluorinated phenylalanine analog and aminoacylate a tRNA, wherein the synthetase has an amino acid sequence of anyone of SEQ ID NO:3-36.
Dependencies are inferred from the claim text.