Gata2 deficient cell line and methods of screening gata2 variants
AssigneeWisconsin Alumni Research Foundation
InventorsEmery Bresnick, Kirby Johnson
Life of this patent
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Abstract
Described herein is a GATA2-deficient human umbilical cord blood-derived erythroid progenitor (HUDEP) cell line, wherein one GATA2 gene copy includes deletion of at least a portion of one or more GATA2 protein-encoding sequences, wherein the deletion results in at least a 25% reduction of GATA2 expression in the GATA2-deficient HUDEP cell line compared to a parent HUDEP cell line without the GATA2 deletion. Also described are methods of screening GATA2 mutations using the GATA2-deficient HUDEP cell lines.
Claims21
A GATA2-deficient human umbilical cord blood-derived erythroid progenitor (HUDEP) cell line, wherein one GATA2 gene copy comprises a deletion of at least a portion of one or more GATA2 protein-encoding sequences, wherein the deletion results in at least a 25% reduction of GATA2 expression in the GATA2-deficient HUDEP cell line compared to a parent HUDEP cell line without the GATA2 deletion.
Dependencies are inferred from the claim text.