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US 2026/0193640 A1Published application
Screening and characterization of binding reagents under equilibrium conditions
AssigneeNautilus Subsidiary, Inc.
InventorKara JUNEAU
Life of this patent
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Abstract
Methods and systems are provided for selecting binding reagents from a library and characterizing selected binding reagents for assays. A selected binding reagent may bind to two or more differing analytes, or epitopes thereof. Further provided are systems that facilitate selection and/or characterization of binding reagents under assay conditions.
Claims18
A method of selecting a binding reagent having a binding specificity for an epitope Θ, comprising:
- (a) providing a plurality of binding targets, wherein each binding target of the plurality of binding targets comprises the epitope Θ, and wherein each binding target of the plurality of binding targets is co-localized with a unique binding target barcode moiety;
- (b) providing a library of binding reagents wherein each binding reagent of the library of binding reagents differs from each other binding reagent of the library of binding reagents with respect to binding specificity, and wherein each binding reagent of the library of binding reagents is attached to a unique binding reagent barcode moiety;
- (c) combining the plurality of binding targets with the library of binding reagents, thereby coupling binding reagents of the library of binding reagents to binding targets of the plurality of binding targets;
- (d) forming a plurality of interaction moieties, wherein each interaction moiety of the plurality of interaction moieties comprises information from a binding target barcode moiety of a binding target of the plurality of binding targets and information from the binding reagent barcode moiety;
- and
- (e) detecting two or more interaction moieties containing information from a same binding reagent barcode moiety, thereby selecting the binding reagent of the library of binding reagents having a binding specificity for the epitope Θ.
3 more elements
A method of characterizing a binding reagent having a binding specificity for an epitope Θ, comprising:
- (a) providing a plurality of binding targets, wherein each binding target of the plurality of binding targets is derived from a proteome, wherein a first fraction of binding targets of the plurality of binding targets comprises the epitope Θ, wherein a second fraction of binding targets of the plurality of binding targets does not comprise the epitope Θ, and wherein each unique binding target of the plurality of binding targets is co-localized with a unique binding target barcode moiety;
- (b) contacting to the plurality of binding targets a plurality of binding reagents, wherein each binding reagent of the plurality of binding reagents is substantially identical, wherein each binding reagent of the plurality of binding reagents has a binding specificity for the epitope Θ, and wherein each individual binding reagent of the plurality of binding reagents is attached to a binding reagent barcode moiety;
- (c) forming a plurality of interaction moieties, wherein each interaction moiety of the plurality of interaction moieties comprises information from a binding target barcode moiety of the plurality of binding targets and information from the binding reagent barcode moiety;
- (d) detecting each interaction moiety of the plurality of interaction moieties;
- and
- (e) determining for each unique binding target barcode moiety a quantity of interaction moieties of the plurality of interaction moieties containing the information from the unique binding target barcode moiety.
3 more elements
-30. (canceled)
A system for characterizing a binding reagent, comprising:
- a) a vessel, wherein the vessel comprises a fluidic medium comprising:
- (i) a plurality of binding reagents;
- and
- (ii) a plurality of binding targets, wherein each binding target of the plurality of binding targets is co-localized with a unique binding target barcode moiety;
- b) a polymer sequencing device;
- and
- c) a fluidic system that is configured to transfer interaction moieties from the vessel to the polymer sequencing device.
4 more elements
Dependencies are inferred from the claim text.