Salt active nuclease compositions and methods
AssigneeNew England Biolabs, Inc.
InventorsCarine Robichon-Iyer, Minyong Chen, Laura Ott
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Abstract
The present disclosure relates, according to some embodiments, to systems, apparatus, compositions, methods, and workflows that include salt active nucleases (e.g., salt active nuclease variants) with desirable properties including, for example, salt tolerance.
Claims30
A salt active nuclease variant having an amino acid sequence that is at least 90% identical to one or more of SEQ ID NOS:1-4, having at its position corresponding to position 52 of SEQ ID NO:1 an amino acid other than Q, and having catalytic activity as an endonuclease and/or an endoribonuclease at a total salt concentration of 250 mM to 1 M.
Dependencies are inferred from the claim text.