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US 2026/0193612 A1Published application
Method of preparing a mass culture of muscle precursor cells (mpcs) and uses thereof
AssigneeUniversitäT ZüRich
InventorsDeana MOHR-HARALAMPIEVA, Jenny Ann PRANGE, Ruud DAS, Marijn DRIESSEN, Emerentius Gerardus ROOSLOOT
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Abstract
Provided are methods of obtaining a mass culture of muscle derived muscle precursor cells (MPCs) using microcarriers as growth substrate, methods for obtaining a therapeutic efficient amount of those cells, a cell population obtained by said methods as well as compositions comprising the expanded cells and methods for preparing a medicament, for example, for use in the treatment of skeletal muscle dysfunctions.
Claims29
A method of obtaining a mass culture of skeletal muscle derived muscle precursor cells (MPCs) comprising at least the following steps:
- (a) cultivating MPCs in a container comprising culture medium which comprises microcarriers under conditions allowing the MPCs to attach to the microcarriers, wherein MPCs are seeded at a density between 500-1500 cells/cm2 growth surface area provided by the microcarriers, preferably at a density between 800-1200 cells/cm2;
- and
- (b) increasing the growth surface area of the culture medium when the cell number has increased about 8-fold to 25-fold;
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- (c) further cultivating the MPCs, preferably until a cell density up to 5-7.5×104 cells/cm2 and/or 4-6.5×105 cells/ml has been reached, and optionally
- (d) harvesting the MPCs.
3 more elements
Dependencies are inferred from the claim text.