Methods of identifying, differentiating between, and/or sorting cells
AssigneeUniversity of Tasmania
InventorLaura Maeve Parsley
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Abstract
The present disclosure relates to methods of identifying, differentiating between and/or sorting sperm cells, or more particularly, X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells, in a sperm sample, the methods comprising determining a level of a Reactive Oxidant Species and/or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample; and based on the determined ROSCC levels, identifying, differentiating between and/or sorting X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells. Also described are enriched or purified X-CBS and Y-CBS cells, and uses thereof.
Claims21
A method of differentiating between X-chromosome bearing sperm (X-CBS) and Y-chromosome bearing sperm (Y-CBS) cells in a sperm sample, the method comprising:
- determining a level of a Reactive Oxidant Species and/or a Cellular Change mediated thereby (ROSCC) in each of a plurality of sperm cells in the sperm sample;
- and
- based on the determined ROSCC levels, differentiating between X-CBS and Y-CBS cells in at least a portion of the plurality of sperm cells.
21-22. (canceled)
Dependencies are inferred from the claim text.