Cell sheet production device and sterile closed circuit for cell sheet production device

A cell sheet production device, to which a sterile closed circuit for a cell sheet production device is to be attached, the sterile closed circuit including

• a sheet forming container provided with a sheet forming solution tank having a plane substrate for cell adhesion for producing a cell sheet,
• a first pipe that is connected to the sheet forming container and injects a cell suspension in which cells are suspended into the sheet forming container,
• a second pipe that is connected to the sheet forming container and injects a culture solution for medium exchange into the sheet forming container, and
• a third pipe that is connected to the sheet forming container and discharges an unnecessary liquid component inside the sheet forming container,
• the cell sheet production device comprising:
• a concentration unit that is connected to the sheet forming container and concentrates the cell suspension;
• a plurality of switching valves provided in a pipe connected to the sheet forming container, the pipe including the first pipe, the second pipe, and the third pipe;
• and
• a liquid transferring mechanism that controls switching of the switching valves, and adjusts a pressure inside the sterile closed circuit for a cell sheet production device via a sterile filter, and transfers a liquid inside the sterile closed circuit for a cell sheet production device based on a difference in the pressure, wherein
• a concentration of the cell suspension is concentrated in the concentration unit, the concentrated cell suspension is injected into the sheet forming container through the first pipe, the cells are seeded on the plane substrate for cell adhesion at a cell seeding density of 3,000 cells/mm2 or more, the unnecessary liquid component is discharged from the third pipe, and the culture solution for medium exchange is injected from the second pipe, thereby producing a cell sheet on the plane substrate for cell adhesion.

A sterile closed circuit for a cell sheet production device, the sterile closed circuit comprising:

• a sheet forming container provided with a sheet forming solution tank having a plane substrate for cell adhesion for producing a cell sheet;
• a first pipe that is connected to the sheet forming container and injects a cell suspension in which cells are suspended into the sheet forming container;
• a second pipe that is connected to the sheet forming container and injects a culture solution for medium exchange into the sheet forming container;
• and
• a third pipe that is connected to the sheet forming container and discharges an unnecessary liquid component inside the sheet forming container, wherein
• a concentration unit that concentrates the cell suspension is connected to the sheet forming container, and
• a concentration of the cell suspension is concentrated in the concentration unit, the concentrated cell suspension is injected into the sheet forming container through the first pipe, the cells are seeded on the plane substrate for cell adhesion at a cell seeding density of 3,000 cells/mm2 or more, the unnecessary liquid component is discharged from the third pipe, and the culture solution for medium exchange is injected from the second pipe, thereby producing a cell sheet on the plane substrate for cell adhesion.

A sterile closed circuit for a cell sheet production device to be attached to a cell sheet production device to produce a cell sheet, the sterile closed circuit comprising:

• a sheet forming container provided with a sheet forming solution tank having a plane substrate for cell adhesion for producing a cell sheet;
• a first pipe that is connected to the sheet forming container and injects a cell suspension in which cells are suspended into the sheet forming container;
• a second pipe that is connected to the sheet forming container and injects a culture solution for medium exchange into the sheet forming container;
• and
• a third pipe that is connected to the sheet forming container and discharges an unnecessary liquid component inside the sheet forming container, wherein
• a concentration unit that concentrates the cell suspension is connected to the sheet forming container,
• a plurality of switching valves are provided in a pipe connected to the sheet forming container, the pipe including the first pipe, the second pipe, and the third pipe,
• a liquid transferring mechanism that controls switching of the switching valves, and adjusts a pressure inside the sterile closed circuit for a cell sheet production device via a sterile filter, and transfers a liquid inside the sterile closed circuit for a cell sheet production device based on a difference in the pressure is provided, and
• a concentration of the cell suspension is concentrated in the concentration unit, the concentrated cell suspension is injected into the sheet forming container through the first pipe, the cells are seeded on the plane substrate for cell adhesion at a cell seeding density of 3,000 cells/mm2 or more, the unnecessary liquid component is discharged from the third pipe, and the culture solution for medium exchange is injected from the second pipe, thereby producing a cell sheet on the plane substrate for cell adhesion.