Method for decelerating the decay-dissociation of c3bbb complex
AssigneeHoffmann-La Roche Inc.
InventorsSebastian FENN, Alexander FIDLER, Anton JOCHNER, Eloed KOERTVELY, Jean-Luc MARY
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Abstract
The present invention relates to a method for stabilizing the C3bBb complex, a rapidly decaying component of the alternative pathway of the complement system, and stabilized C3bBb complexes and uses thereof.
Claims16
A method for providing antibodies specifically binding to C3bBb complex, comprising:
- a) providing a C3bBb complex using recombinant human factor B (FB) comprising a D279G mutation, and
- b) screening and selecting an antibody that specifically binds to the C3bBb complex provided in step a).
A method of decelerating the decay-dissociation of the C3bBb complex, including providing C3bBb complex using recombinant FB comprising a D279G mutation.
A method of providing a stabilized C3bBb complex, comprising contacting human Complement C3 beta chain (C3b) with recombinant human FB in aqueous solution, under subsequent addition of human Factor D (FD), and optionally subsequent purification of C3bBb, characterized in that the recombinant human FB comprises a D279G mutation.
The method according to any one of the preceding claims, wherein the C3bBb complex is formed by contacting human Complement C3 beta chain (C3b) with recombinant human FB comprising a D279G mutation in aqueous solution, under subsequent addition of human Factor D (FD) and subsequent purification of C3bBb.
The method according to any one of the preceding claims, wherein the C3bBb complex is provided by contacting C3b, FB and FD in aqueous solution comprising magnesium ions, preferably Mg2+ ions, more preferably MgCl2.
A recombinant human Factor B protein comprising a D279G mutation.
A recombinant human Factor B protein comprising D279G, K350N and/or M458I mutations.
A stabilized C3bBb complex comprising a Bb subunit of a recombinant human FB with a D279G mutation, optionally further comprising a K350N and/or a M458I mutation.
Dependencies are inferred from the claim text.