Compositions and methods for treatment of netherton syndrome with lekti expressing recombinant microbes
AssigneeAzitra Inc
InventorsTravis Michael Whitfill, Azim Momin Munivar
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Abstract
The present disclosure provides, inter alia, engineered microbes expressing recombinant LEKTI domains that are effective to treat or ameliorate the symptoms of Netherton Syndrome. In certain embodiments, compositions, methods, and kits are provided comprising LEKTI domain expressing microbes.
Claims22
A composition for the treatment of a skin disease comprising:
- a microbe genetically modified to express and provide one or more LEKTI protein domains onto the skin of a mammal, wherein the LEKTI protein domains are effective to penetrate one or more layers of the mammal's skin and effective to inhibit serine protease activity of at least one serine protease in or on the mammal's skin.
A method of treating or ameliorating the effects of a skin disease of a mammal in need thereof comprising:
- providing onto a surface of the skin of the mammal a microbe genetically modified to express one or more LEKTI protein domains, wherein the LEKTI protein domains are effective to penetrate one or more layers of the mammal's skin and effective to inhibit activity of at least one serine protease in or on the mammal's skin.
A kit for the treatment or amelioration of the effects of a skin disease of a mammal in need thereof comprising:
- (1) a composition comprising a microbe that is genetically modified to express one or more LEKTI protein domains, wherein the LEKTI protein domains are effective to penetrate one or more layers of the mammal's skin and effective to inhibit serine protease activity of at least one serine protease in or on the mammal's skin;
- and
- (2) reagents for applying the composition to the skin of the mammal.
A composition for the treatment of skin disease comprising a microbe comprising pJB38-LEKTI-complete plasmid construct.
A composition comprising pJB38-LEKTI-complete plasmid construct.
A recombinant microorganism capable of secreting a polypeptide, wherein the recombinant microorganism comprises an expression vector comprising a first coding sequence comprising a gene capable of expressing the polypeptide and a second coding sequence comprising a gene capable of expressing a cell penetrating peptide.
Dependencies are inferred from the claim text.