Sample Solution Separation Method, Sample Solution Separation Device, and Sample Solution Separation Apparatus

A sample solution separation method in a microdevice for biological sample analysis, the method comprising the steps of:

• introducing a sample solution into a plurality of fractions included in the microdevice for biological sample analysis;
• introducing a liquid photocurable resin composed of a monomer material into a flow path of the microdevice for biological sample analysis and covering openings of the plurality of fractions with the liquid photocurable resin to separate the plurality of fractions into which the sample solution has been introduced;
• and
• irradiating the microdevice for biological sample analysis into which the liquid photocurable resin has been introduced with light to solidify the liquid photocurable resin,
• wherein the microdevice for biological sample analysis is used for digital PCR or real-time PCR.

A sample solution separation device used for biological sample analysis, the device comprising:

• a substrate;
• an inlet portion formed in the substrate for introducing a sample solution;
• an outlet portion formed in the substrate for discharging at least an excess sample solution;
• a plurality of fractions formed in the substrate and holding a sample solution to be introduced;
• and
• a photocurable resin storing unit formed in the substrate that stores a liquid photocurable resin composed of a monomer material,
• wherein a pressure application hole for applying pressure for discharging the stored liquid photocurable resin from the photocurable resin storing unit is formed in the photocurable resin storing unit, and
• the device is used for digital PCR or real-time PCR.

A sample solution separation apparatus configured to separate a sample solution introduced into a plurality of fractions of a microdevice for biological sample analysis, the apparatus comprising:

• a device mounting unit on which the microdevice for biological sample analysis is mounted;
• a photocurable resin storing unit configured to store a liquid photocurable resin composed of a monomer material, which is to be introduced into the microdevice for biological sample analysis after the sample solution is introduced into the plurality of fractions;
• a pressure application device configured to apply pressure to the photocurable resin storing unit and introduce the liquid photocurable resin into the microdevice for biological sample analysis;
• and
• a light irradiation unit configured to irradiate the liquid photocurable resin introduced into the microdevice for biological sample analysis with light,
• wherein the microdevice for biological sample analysis is used for digital PCR or real-time PCR.