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AssigneeMusculoskeletal Transplant Foundation
InventorsMichael Zbigniew Kubik, Marc Long, Eric J. Semler, Maitri Kapadia
Estimating the term…
Devitalized tissue-derived compositions contain extracellular matrix (ECM), devitalized endogenous cells and/or one or more endogenous bioactive substances (e.g., cellular components, including extracellular vesicles (e.g., exosomes)) and are capable of storage at room temperature. Because native cells are devitalized, but intentionally not removed from these compositions, beneficial proteins and other substances contained in the devitalized endogenous cells and endogenous extracellular vesicles are retained in the compositions and available and accessible in quantities comparable to viable cryopreserved tissue-derived compositions and greater than conventional decellularized tissue-derived graft materials. Lyophilizing renders the devitalized tissue-derived compositions capable of storage at ambient temperatures, without the need for freezing or refrigeration. Methods for producing and using the devitalized tissue-derived compositions, either alone or with other materials and substances are also provided. In exemplary embodiments, bone tissue samples are subjected to initial freeze-thaw cycles to devitalize, but retain, endogenous cells and extracellular vesicles contained therein.
A devitalized tissue-derived composition derived from one or more tissue samples which include one or more tissue types, the devitalized tissue-derived composition comprising:
A method for producing a devitalized tissue-derived composition which comprises one or more endogenous bioactive substances which were retained from one or more tissue samples processed to produce the devitalized tissue-derived composition and, optionally, a population of devitalized endogenous cells which were retained from the one or more tissue samples and which are undisrupted and resident in the ECM, the method comprising the steps of:
A method for producing devitalized tissue-derived compositions from one or more tissue samples comprising a cancellous bone sample comprising the steps of:
Dependencies are inferred from the claim text.