Methods for treating liver cancer by genomic targeting
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorJianhua LUO
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods of treating patients having liver cancer by performing a genome-targeting technique. It is based, at least in part, on the discovery that a genome editing technique that specifically targets genomic mutations can induce cell death in a cancer cell, e.g., a hepatocellular cancer cell, having the genomic gene. The present disclosure provides methods for treating cancer patients that include performing a genome editing technique targeting a genomic mutation present within one or more cells of a subject to produce an anti-cancer effect.
Claims20
A genome editing method comprising:
- (i) introducing a first polynucleotide encoding a suicide gene and a second polynucleotide encoding a nuclease into a cell, wherein the nuclease targets a genomic mutation of a gene selected from CTNNB1, SLTM, SLC45A2, AMACR, or a combination thereof, and
- (ii) contacting the cell with an agent capable of inducing killing of the cell.
A genome editing system comprising:
- (i) a first polynucleotide encoding a suicide gene;
- (ii) a second polynucleotide encoding a nuclease targeting a genomic mutation of a gene selected from CTNNB1, SLTM, SLC45A2, AMACR, or a combination thereof;
- and
- (iii) a therapeutically effective amount of an agent to induce the killing of a cell expressing the suicide gene.
A method of treating a subject having a pre-malignant or neoplastic condition, the method comprising:
- (i) detecting, in a sample obtained from the subject, a genomic mutation of a gene selected from CTNNB1, SLTM, SLC45A2, AMACR, or a combination thereof,
- (ii) administering an effective amount of a first polynucleotide encoding a suicide gene and a second polynucleotide encoding a nuclease targeting the genomic mutation;
- and
- (iii) administering to the subject an effective amount of an agent to induce the killing of a cell expressing the suicide gene.
Dependencies are inferred from the claim text.