Use of conditioned medium derived from cultivation of mesenchymal stem cells of the umbilical cord for inducing, stimulating and promoting hair growth and regeneration

A non-therapeutic method of alleviating and/or reducing hair loss and/or hair thinning, wherein the method comprises treating the hair of a subject with conditioned medium derived from cultivation of a mesenchymal stem cell population of the umbilical cord, wherein the mesenchymal stem cell population is a mesenchymal stem cell population of the amniotic membrane of the umbilical cord or a mesenchymal stem population of the Wharton's jelly of the umbilical cord,

• wherein the conditioned medium is obtained by
• (a) cultivating the mesenchymal stem cells of the umbilical cord in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum),
• wherein the culture medium comprises DMEM in a final concentration of 55 to 65% (v/v), F12 in a final concentration of 5 to 15% (v/v), M171 in a final concentration of 15 to 30% (v/v) and FBS in a final concentration of 1 to 8% (v/v).
• (b) removing the mesenchymal stem cells of the umbilical cord from the culture medium;
• and cultivating the mesenchymal stem cells of the umbilical cord in a further culture medium, optionally wherein the further cell culture medium comprises a water-soluble antioxidant,
• wherein the further medium is a basal medium suitable for cultivation of mesenchymal stem cells, and
• (c) collecting the further cell culture medium.

A method of producing a conditioned medium, the method comprising

• (a) cultivating mesenchymal stem cells of the umbilical cord, wherein the mesenchymal stem cells of the umbilical cord are derived from a mesenchymal stem cell population of the amniotic membrane of the umbilical cord or from a mesenchymal stem population of the Wharton's jelly of the umbilical cord, in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum), wherein the culture medium comprises DMEM in a final concentration of 55 to 65% (v/v), F12 in a final concentration of 5 to 15% (v/v), M171 in a final concentration of 15 to 30% (v/v) and FBS in a final concentration of 1 to 8% (v/v);
• (b) removing the mesenchymal stem cells of the umbilical cord from the culture medium;
• and
• (c) cultivating the mesenchymal stem cells of the umbilical cord in a further culture medium, wherein the further medium is a basal medium suitable for cultivation of mesenchymal stem cells, and wherein the further cell culture medium comprises a water-soluble antioxidant,
• wherein the conditioned medium is obtained by collecting the further cell culture medium.

The use of conditioned medium derived from cultivation of a mesenchymal stem cell population of the umbilical cord for the manufacture of a medicament for inducing, stimulating and/or promoting hair growth and/or hair regeneration;

• and/or for alleviating and/or reducing hair loss and/or hair thinning of a subject,
• wherein the mesenchymal stem cell population is a mesenchymal stem cell population of the amniotic membrane of the umbilical cord or a mesenchymal stem population of the Wharton's jelly of the umbilical cord,
• wherein the conditioned medium is obtained by
• a) cultivating the mesenchymal stem cells of the umbilical cord in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum),
• wherein the culture medium comprises DMEM in a final concentration of 55 to 65% (v/v), F12 in a final concentration of 5 to 15% (v/v), M171 in a final concentration of 15 to 30% (v/v) and FBS in a final concentration of 1 to 8% (v/v).
• b) removing the mesenchymal stem cells of the umbilical cord from the culture medium;
• and cultivating the mesenchymal stem cells of the umbilical cord in a further culture medium, optionally wherein the further cell culture medium comprises a water-soluble antioxidant,
• wherein the further medium is a basal medium suitable for cultivation of mesenchymal stem cells, and
• c) collecting the further cell culture medium.