Application of super-resolution imaging technology in visual evaluation of pancreatic b-cell changes

The application of super-resolution imaging technology in visual evaluation of pancreatic β-cell changes includes the following steps:

• S1. Optimize super-resolution imaging technology to achieve precise localization of microbubbles at lower frame rates and higher concentrations, enabling super-resolution ultrasound imaging based on clinical diagnostic equipment.
• S2. Utilize motion correction techniques to overcome the influence of displacement caused by respiration and heartbeat, thereby enhancing the accuracy of microvascular imaging.
• S3. Visualization of organ microvascular distribution through super-resolution imaging technology, facilitating the construction of flow velocity maps, directional maps, etc., achieving micron-level resolution;
• S4. Employ super-resolution imaging technology to accurately quantify microvascular morphology and functional change parameters, including distortion, fractal dimension, blood flow velocity, vessel density, and vessel diameter.
• S5. Perform three-dimensional reconstruction of microvascular pathological staining results from the model as the gold standard to verify the accuracy of super-resolution imaging technology in evaluating β-cell function and quality.