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US 2026/0185145 A1Published application
Circularizable probes for in situ analysis
Assignee10x Genomics, Inc.
InventorsFelice Alessio BAVA, Jorge Iván HERNÁNDEZ NEUTA, Malte KÜHNEMUND, Jessica ÖSTLIN, Xiaoyan QIAN, Toon VERHEYEN
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Abstract
The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, provided herein are methods and compositions for detecting a region of interest in a target nucleic acid.
Claims20
A method for analyzing a biological sample, the method comprising:
- (a) contacting the biological sample comprising a target nucleic acid with a first nucleic acid molecule, a second nucleic acid molecule, and a third nucleic acid molecule that each hybridizes to the target nucleic acid molecule,
- (b) using the first nucleic acid molecule as a template to perform a first ligation of the second and third nucleic acid molecules;
- and
- (c) using the target nucleic acid as a template to perform an additional ligation of the second and third nucleic acid molecules;
- thereby generating a circularized probe comprising the second and third nucleic acid molecules.
2 more elements
Dependencies are inferred from the claim text.