Pyridoxal kinase mutant
AssigneesTaizhou University, Taizhou Lingfeng Biotechnology Co., Ltd.
InventorsZhongyi YANG, Fei WANG, Qingfeng CAI, Yi WEI, Weijin HUANG
Life of this patent
Estimating the term…
Abstract
A pyridoxal kinase mutant is provided. To solve the problem of low concentration of existing enzyme catalyzed products, provided is a pyridoxal kinase mutant having an amino acid sequence selected from an amino acid sequence as shown in SEQ ID NO: 1 in which lysine at position 229 is mutated to alanine, phenylalanine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, aspartic acid, or glutamic acid; and the pyridoxal kinase mutant is used to catalyze the synthesis of pyridoxal phosphate from pyridoxal.
Claims14
A pyridoxal kinase mutant, wherein the pyridoxal kinase mutant has an amino acid sequence selected from the amino acid sequence as shown in SEQ ID NO:
- 1 in which lysine at position 229 is mutated to alanine, phenylalanine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, aspartic acid, or glutamic acid;
- and the pyridoxal kinase mutant is used to catalyze a synthesis of pyridoxal phosphate from pyridoxal.
A microbial cell having a microorganism as an expression host, wherein the microorganism carries a pyridoxal kinase mutant or carries a recombinant expression vector containing the pyridoxal kinase mutant;
- the pyridoxal kinase mutant has an amino acid sequence selected from the amino acid sequence as shown in SEQ ID NO:
- 1 in which lysine at position 229 is mutated to alanine, phenylalanine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, aspartic acid, or glutamic acid;
- and the pyridoxal kinase mutant is used to catalyze a synthesis of pyridoxal phosphate from pyridoxal.
Dependencies are inferred from the claim text.