Production method for genome-edited cells
AssigneeKyushu University, National University Corporation
InventorsAtsushi SUZUKI, Masaki KAWAMATA
Life of this patent
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Abstract
A production method for a cell in which only one allele is genome-edited includes: a step of introducing, into a cell, (A) at least one selected from the group consisting of (a1) a guide RNA in which one or more nucleotide residues are added to a 5′-end of a spacer sequence, (a2) a guide RNA containing a spacer sequence having single-base or multiple-base mismatches with respect to a target sequence, and (a3) an expression vector that can cause the guide RNA of (a1) or (a2) to be expressed, and (B) at least one selected from the group consisting of a Cas protein and an expression vector that can cause the Cas protein to be expressed.
Claims19
A production method for a cell in which only one allele is genome-edited, the production method comprising:
- introducing, into a cell,
- (A) at least one selected from the group consisting of (a1) a guide RNA in which 5 or more nucleotide residues are added to a 5′-end of a spacer sequence, and (a3) an expression vector for the guide RNA of (a1), and
- (B) at least one selected from the group consisting of a Cas protein and an expression vector for the Cas protein.
7-16. (canceled)
A method for inhibiting cytotoxicity caused by genome editing, the method comprising:
- introducing, into a cell,
- (A) at least one selected from the group consisting of (a1) a guide RNA in which 5 or more nucleotide residues are added to a 5′-end of a spacer sequence, and (a3) an expression vector for the guide RNA of (a1), and
- (B) at least one selected from the group consisting of a Cas protein and an expression vector for the Cas protein.
A method for increasing homologous directed repair efficiency induced by genome editing, the method comprising:
- introducing, into a cell,
- (A) at least one selected from the group consisting of (a1) a guide RNA in which 5 or more nucleotide residues are added to a 5′-end of a spacer sequence, and (a3) an expression vector for the guide RNA of (a1), and
- (B) at least one selected from the group consisting of a Cas protein and an expression vector for the Cas protein.
Dependencies are inferred from the claim text.