CCA Gene For Virus Resistance
OwnerJonathan KALISVAART, Raoul Jacobus Johannes Maria FRIJTERS +2inventors
InventorsJonathan KALISVAART, Raoul Jacobus Johannes Maria FRIJTERS, Daniël Johannes Wilhelmus LUDEKING, Alwin Johannes Marinus ROOVERS
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Abstract
The present invention relates to a modified CCA gene which encodes a CCA-adding enzyme, which modified CCA gene leads to resistance against a positive-strand RNA virus having a transfer RNA-like structure (TLS). The invention further relates to plants and seeds comprising the modified genes, methods for making and identifying such plants and use of the gene.
Claims32
An agronomically elite tomato (Solanum lycopersicum) plant comprising a modified CCA gene which encodes a CCA-adding enzyme, which modified CCA gene leads to resistance against a positive-strand RNA virus having a TLS, wherein the modified CCA gene is selected from:
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme according to SEQ ID NO:
- 8;
- a gene comprising a promoter sequence comprising SEQ ID NO:
- 12;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 2;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 8;
- or
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8, wherein the CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8 comprises a N535D substitution.
A method for the production of a plant which is resistant to a positive-strand RNA virus comprising a TLS, said method comprising:
- a) crossing a first parent plant, with a second parent plant;
- b) optionally performing one or more rounds of selfing and/or crossing of the plant resulting from the cross in step a) to obtain a further generation population;
- c) selecting from the plant resulting from the cross in step a), or from the further generation population of step b), a plant that comprises a modified CCA gene, wherein the selected plant is resistant to a positive-strand RNA virus comprising a TLS;
- and
- wherein the modified CCA gene is selected from:
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme according to SEQ ID NO:
- 8;
- a gene comprising a promoter sequence comprising SEQ ID NO:
- 12;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 2;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 8;
- or
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8, wherein the CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8 comprises a N535D substitution.
A method for the production of a Solanum lycopersicum plant resistant to ToBRFV, said method comprising:
- a) crossing a first parent plant, with a second parent plant;
- b) optionally performing one or more rounds of selfing and/or crossing of the plant resulting from the cross in step a) to obtain a further generation population;
- c) selecting from the plant resulting from the cross in step a), or from the further generation population of step b), a plant that comprises a modified CCA gene, wherein the selected plant is resistant to ToBRFV;
- and
- wherein the modified CCA gene is selected from:
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme according to SEQ ID NO:
- 8;
- a gene comprising a promoter sequence comprising SEQ ID NO:
- 12;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 2;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 8;
- or
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8, wherein the CCA-adding enzyme having at least 90% sequence identity to SEQ ID NO:
- 8 comprises a N535D substitution.
Dependencies are inferred from the claim text.