Systems and methods for biomolecule retention
AssigneeNautilus Subsidiary, Inc.
InventorsTorri Elise RINKER, Christina INMAN, Parag MALLICK, Steven TAN, Pengyu HAO, Elvis IKWA, Pierre INDERMUHLE, Tural AKSEL, Stephen HENDRICKS, Sadie INGLE
Life of this patent
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Abstract
Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.
Claims34
-30. (canceled)
A method of attaching an analyte to a solid support, comprising:
- (a) providing a nucleic acid particle, wherein the nucleic acid particle is hybridized to a first oligonucleotide, wherein the first oligonucleotide comprises a first functional group and a second functional group;
- (b) attaching the analyte to the first functional group of the first oligonucleotide;
- (c) attaching the second functional group to the solid support;
- and
- (d) after attaching the second functional group to the solid support, dissociating the nucleic acid particle from the first oligonucleotide.
A method of forming an array of analytes, comprising:
- (a) providing a solid support comprising a plurality of sites, wherein a plurality of nucleic acid particles is attached to the plurality of sites, wherein each site of the plurality of sites is attached to only one nucleic acid particle of the plurality of nucleic acid particles, wherein a plurality of analytes is attached to the plurality of nucleic acid particles, wherein each nucleic acid particle of the plurality of nucleic acid particles is attached to only one analyte of the plurality of analytes, wherein each nucleic acid particle is individually hybridized to a first oligonucleotide, wherein each first oligonucleotide individually comprises a first attachment moiety and a second attachment moiety, wherein each first attachment moiety is attached to the only one analyte, and wherein each second attachment moiety is attached to the solid support;
- and
- (b) dissociating each nucleic acid particle from each first oligonucleotide, thereby providing the plurality of analytes attached to the plurality of sites.
A composition, comprising:
- (a) a nucleic acid particle;
- (b) a first oligonucleotide, wherein the first oligonucleotide is hybridized to the nucleic acid particle, wherein the first oligonucleotide comprises a first functional group and a second functional group;
- (c) an analyte, wherein the analyte is attached to the first functional group;
- and
- (d) a solid support, wherein the solid support is attached to the second functional group of the first oligonucleotide.
Dependencies are inferred from the claim text.