Completely noninvasive multi-analyte monitoring system for cell culture processes
OwnerGovind RAO, Michael TOLOSA +3inventors
InventorsGovind RAO, Michael TOLOSA, Vida RAHMATNEJAD, Vikash KUMAR, Xudong GE
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Abstract
A noninvasive system and method for simultaneous monitoring of species in a cell culture medium is described. Instead of direct contact with the culture media, the measurements can be made through permeable membranes via either a port in the culture vessel wall or a port in a flow cell. The noninvasive monitoring system and method can offer accurate, and contamination-minimized monitoring of critical process parameters including dissolved O2, pH, and dissolved CO2. These advancements will enhance the control and optimization of cell culture processes, promising improved cell culture performance.
Claims34
A system for noninvasively detecting and quantitating species within a container, said system comprising:
- the container, wherein the container comprises at least one hole or port in a wall of the container, wherein a membrane covers, or is positioned within, each hole or port, and wherein the membrane permits the passage of the species to be detected and quantitated therethrough;
- at least one sensing device, wherein the at least one sensing device is positioned on, or in proximity to, the membrane, wherein the at least one sensing device is not in contact with any fluid present in the container, and wherein the at least one sensing device collects the species that pass through the membrane, reacts with the species that pass through the membrane, or both;
- and
- a detection device for quantitating the species collected in, reacted with, or both collected in and reacted with, the at least one sensing device.
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A flow cell for noninvasively detecting and quantitating species, said flow cell comprising:
- a layer for positioning of a first sensing device;
- a first membrane layer, wherein the first membrane layer permits the passage of a species to be detected by the first sensing device;
- a layer comprising a media channel;
- a second membrane layer, wherein the second membrane layer permits the passage of a species to be detected by a second sensing device;
- and
- a layer for positioning of the second sensing device,
- wherein the layer comprising the media channel can be communicatively connected to a vessel comprising a medium, wherein the medium comprises species to be detected and quantitated.
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A system for noninvasively detecting and quantitating species in a medium contained in a vessel, said system comprising:
- a flow cell;
- a pump;
- and
- a vessel,
- wherein the flow cell is communicatively connected to the vessel comprising the medium for detection and quantitation of a species contained therein, and wherein the flow cell comprises:
- a flow cell container, wherein the flow cell container comprises at least one hole or port in a wall of the flow cell container, an inlet and an outlet, wherein a membrane covers, or is positioned within, each hole or port, and wherein the membrane permits the passage of the species to be detected and quantitated therethrough;
- at least one sensing device, wherein the at least one sensing device is positioned on, or in proximity to, the membrane, wherein the at least one sensing device collects the species that pass through the membrane, reacts with the species that pass through the membrane, or both;
- and
- a detection device for quantitating the species collected in, reacted with, or both collected in and reacted with, the at least one sensing device.
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Dependencies are inferred from the claim text.