Anti-pcrv antibodies that bind pcrv, compositions comprising anti-pcrv antibodies, and methods of use thereof
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsChristos Kyratsous, Alida Coppi
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Abstract
The present disclosure provides antibodies and antigen-binding fragments of antibodies that bind to Pseudomonas aeruginosa PcrV, and methods of using the same. According to certain embodiments, the disclosure includes antibodies and antigen-binding fragments of antibodies that bind PcrV. The anti-PcrV antibodies and antigen-binding fragments are useful for the prevention and treatment of P. aeruginosa infections.
Claims73
-49. (canceled)
A set of polynucleotide molecules comprising:
- a first polynucleotide molecule comprising a polynucleotide sequence that encodes a heavy chain variable region (HCVR) of an antibody or antigen-binding fragment thereof, and
- a second polynucleotide molecule comprising a polynucleotide sequence that encodes a light chain variable region (LCVR) of said antibody or antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof comprises:
- three heavy chain complementarity determining regions (CDRs) (HCDR1, HCDR2 and HCDR3) contained within any one of the HCVR sequences selected from the group consisting of SEQ ID NOs:
- 34, 50, 2, and 18;
- and
- three light chain CDRs (LCDR1, LCDR2 and LCDR3) contained within any one of the LCVR sequences selected from the group consisting of SEQ ID NOs:
- 42, 58, 10, and 26.
A set of vectors comprising:
- a first vector comprising a polynucleotide sequence that encodes an HCVR of an antibody or antigen-binding fragment thereof that specifically binds to P. aeruginosa PcrV, and
- a second vector comprising a polynucleotide sequence that encodes an LCVR of said antibody or antigen-binding fragment thereof,
- wherein the antibody or antigen-binding fragment thereof comprises:
- three heavy chain CDRs (HCDR1, HCDR2 and HCDR3) contained within any one of the HCVR sequences selected from the group consisting of SEQ ID NOs:
- 34, 50, 2, and 18;
- and
- three light chain CDRs (LCDR1, LCDR2 and LCDR3) contained within any one of the LCVR sequences selected from the group consisting of SEQ ID NOs:
- 42, 58, 10, and 26.
The set of vectors of claim of 72, wherein the antibody or antigen-binding fragment thereof comprises an HCVR/LCVR amino acid sequence pair selected from the group consisting of SEQ ID NOs:
- 34/42, 50/58, 2/10, and 18/26.
A method of producing an antibody or antigen-binding fragment thereof that specifically binds to P. aeruginosa PcrV comprising culturing a host cell under conditions permitting production of the antibody or antigen-binding fragment thereof, and recovering the antibody or antigen-binding fragment thereof so produced,
- wherein the host cell comprises a polynucleotide molecule, or a vector comprising a polynucleotide molecule, and
- wherein said polynucleotide molecule comprises a polynucleotide sequence that encodes an HCVR and a polynucleotide sequence that encodes an LCVR of the antibody or antigen-binding fragment thereof, wherein the antibody or antigen-binding fragment thereof comprises:
- three heavy chain CDRs (HCDR1, HCDR2 and HCDR3) contained within any one of the HCVR sequences selected from the group consisting of SEQ ID NOs:
- 34, 50, 2, and 18;
- and
- three light chain CDRs (LCDR1, LCDR2 and LCDR3) contained within any one of the LCVR sequences selected from the group consisting of SEQ ID NOs:
- 42, 58, 10, and 26.
Dependencies are inferred from the claim text.