Method for simultaneously preparing chlorophyll paste and 1-deoxynojirimycin

Abstract

A method for simultaneously preparing a chlorophyll paste and 1-deoxynojirimycin includes the following steps: (1) drying a silkworm excrement in a shaded area until the silkworm excrement reaches a specific moisture content; (2) placing the silkworm excrement into an extraction tank, adding an extraction solvent to obtain a mixture, and stirring the mixture with a water bath, filtering the mixture, the extraction solvent comprising both a non-polar solvent and a polar solvent; (3) subjecting the mixture to a coarse filtration to obtain a crude filtrate; (4) subjecting the crude filtrate to centrifugation, obtaining a chlorophyll-rich light phase and a 1-deoxynojirimycin-rich heavy phase; and (5) undergoing fine filtration and concentration with the chlorophyll-rich light phase to obtain a chlorophyll paste, and subjecting the 1-deoxynojirimycin-rich heavy phase with fine filtration, concentration, formulation, and spray drying to prepare 1-deoxynojirimycin.

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