Improved salmonella vectored therapies for treatment of cancer
OwnerRoy CURTISS, III, Shifeng WANGinventors
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Abstract
A genetically modified Salmonella cell (GMSC) engineered to exhibit specific targeting to cells and regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by a target cell in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.
Claims28
A genetically modified Salmonella cell (GMSC) engineered to exhibit regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by target cells in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization.
(canceled)
A genetically modified Salmonella cell (GMSC) engineered to exhibit regulated delayed lysis in vivo, the GMSC comprising a first heterologous nucleic acid that encodes a first gene product that causes the GMSC to be selectively localized to and/or internalized by target cells in vivo and a second heterologous nucleic acid that encodes a second gene product that facilitates killing of the target cells following internalization;
- wherein expression of the first heterologous nucleic acid is controlled by a bacterial promoter, optionally further comprising a sequence for secretion of the first gene product;
- and wherein expression of the second heterologous nucleic acid is controlled by a eukaryotic promoter for delivery to the nucleus of the target cells, wherein the bacterial promoter optionally comprises Ptrc, Ptac, Plac, or Plpp, wherein the sequence for secretion optionally comprises bla SSopt and wherein the eukaryotic promoter optionally comprises PCMV or PEF1α.
28-45. (canceled)
Dependencies are inferred from the claim text.