US 2026/0177781 A1 · IMMERSION OBJECTIVE, OPTICAL SYSTEM AND DETECTION METHOD

Abstract

An immersion objective, an optical system and a detection method. The immersion objective sequentially comprises, in the direction from an object side (S) to an image side, a first lens group (G1) having a positive focal power, at least one group of lens groups (G5, G6) having a negative focal power, and an immersion medium (m) which is located on the object side (S) of the first lens group (G1) and immerses at least part of the first lens group (G1), wherein the working distance S0 of the immersion medium (m) between the first lens group (G1) and the object side (S) and the focal length Fobj of the immersion objective satisfy the conditional expression 0≤S0/Fobj≤0.16. By means of the cooperation of a plurality of groups of lenses, an immersion objective has a relatively small size and a relatively low mass within a relatively compact size range, and a large field of view, a high resolution and a low distortion are achieved, thereby improving the optical performance of the immersion objective.

Claims27

Dependent claims

An immersion objective, comprising:

sequentially along a direction from an object space to an image space, a first lens group with positive focal power, at least one lens group with negative focal power and an immersion medium, wherein the immersion medium is located on an object side of the first lens group, at least part of the first lens group is immersed in the immersion medium, and a working distance S0 of the immersion medium located between the first lens group and the object space and a focal length Fobj of the immersion objective satisfy a conditional expression:
0
<
S
0
/
F
obj
≤
0.16

7 more elements

A nucleic acid detection system, comprising a sequencing chip and an optical system, wherein the sequencing chip is configured to support a to-be-detected sample, and the optical system comprises an objective and an immersion medium arranged between the objective and the to-be-detected sample, and wherein an end of the objective close to the to-be-detected sample is immersed in the immersion medium, and a working distance S0 of the immersion medium between the objective and the to-be-detected sample and a focal length Fobj of the objective satisfy a conditional expression:

0
<
S
0
/
F
obj
≤
0.16
.

7 more elements

A biochemical detection method, comprising:

loading a to-be-detected sample into a lane of a chip flow cell;
and
loading a reagent with a plurality of different reaction components into the lane of the chip flow cell to perform a biochemical reaction between the to-be-detected sample and the reagent, wherein
the reaction components comprise at least one of:
a sample generation component and a sample analysis component;
optionally, the biochemical reaction comprises generating a sample in the lane of the chip flow cell, which comprises:
directing different sample generation components to flow into the lane and controlling reaction conditions in the lane to generate the sample;
and
the biochemical reaction comprises analyzing the sample in the lane, which comprises:
directing the sample analysis component to flow into the lane, wherein the sample analysis component reacts with the sample to generate a detectable signal,
wherein the biochemical detection method further comprises:
identifying the detectable signal using an optical system, wherein the optical system comprises an objective and an immersion medium arranged between the objective and the to-be-detected sample, and a working distance S0 of the immersion medium located between the objective and the to-be-detected sample and a focal length Fobj of the objective satisfy a conditional expression:
0
<
S
0
/
F
obj
≤
0.16
.

19 more elements

Dependencies are inferred from the claim text.

Immersion objective, optical system and detection method

Life of this patent

Immersion objective, optical system and detection method

Life of this patent

Abstract

Claims27