Apparatus and methods for fabrication of nanopatterned arrays
AssigneeUniversity of Wyoming
InventorsCaleb HILL, Jeffrey HALPERN
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Abstract
A biosensor for monitoring surface binding events is disclosed. The biosensor comprises an array of nanoparticles and an analyte responsive polymer. The array of nanoparticles includes a plurality of nanoparticles distributed across the nanoparticle array. The analyte responsive polymer includes a recognition element at a first end of the polymer and a terminus at a second end of the polymer distal from the recognition element, the terminus end being conjugated to the nanoparticles in the array. When the recognition element reacts with an analyte, the analyte responsive polymer creates an electrochemical signal at the surface of the nanoparticle array which can be measured to monitor surface events of the analyte responsive polymer.
Claims20
A method of fabricating an array of nanoparticles, comprising:
- synthesizing a plurality of nanoparticles;
- forming a solution of nanoparticles and solvent;
- loading the solution into a pipet;
- bringing the pipet into contact with a substrate at an array of contact points;
- depositing an array of solution droplets at the corresponding array of contact points, wherein each of the solution droplets contains a nanoparticle;
- and
- evaporating the solvent from the solution droplets to form an array of nanoparticles.
A method of fabricating an array of nanoparticles, comprising:
- synthesizing a plurality of nanoparticles;
- forming a solution of nanoparticles and solvent;
- loading the solution into a pipet coupled to a positioning system;
- bringing the pipet into contact with a substrate at an array of contact points using the positioning system;
- depositing an array of solution droplets at the corresponding array of contact points on the substrate using the positioning system, wherein each of the solution droplets contains a nanoparticle, the depositing comprising:
- contacting the substrate using a first piezo coupled to the pipet;
- detecting the contact between the pipet and the substrate using a pressure-induced electrical output of the first piezo and a second piezo coupled to the substrate;
- and
- releasing the contact between the pipet and the substrate when a threshold of the pressure-induced electrical output is reached to deposit a solution droplet of the array of solution droplets;
- and
- evaporating the solvent from the solution droplets to form an array of nanoparticles.
An apparatus, comprising:
- a positioning system, comprising:
- a first piezo;
- and
- a second piezo;
- a pipet coupled to the first piezo;
- and
- a substrate coupled to the second piezo, wherein the positioning system is configured to locally address the pipet to the substrate with a solution containing a nanoparticle.
Dependencies are inferred from the claim text.