US 2026/0177544 A1Published application

Method for detecting test substance in specimen

AssigneeTokyo Metropolitan Institute for Geriatrics and Gerontology

InventorsTetsuya KIMURA, Katsumi KAWANO

Life of this patent

Estimating the term…

Loading patent…

Abstract

An object of the present invention is to provide a highly sensitive method for detecting a biomarker. The object can be achieved by a method for detecting a test substance in a specimen of the present invention, the method being (A) a sandwich method including: (1) bringing a specimen into contact with a magnetic particle on which a primary substance is immobilized and which has a particle diameter that does not cause interference with a wavelength for measurement with a laser, to form a primary substance-test substance complex of the primary substance and a test substance in the specimen; (2) bringing the primary substance-test substance complex into contact with a secondary substance, to form a primary substance-test substance-secondary substance complex of the primary substance-test substance complex and the secondary substance; and (3) detecting the primary substance-test substance-secondary substance complex on the magnetic particle with a laser; or (B) a competitive method including: (1) bringing a specimen and a secondary substance into contact with a magnetic particle on which a primary substance is immobilized and which has a particle diameter that does not cause interference with a wavelength for measurement with a laser, to form a primary substance-secondary substance complex of the primary substance and the secondary substance; and (2) detecting the primary substance-secondary substance complex on the magnetic particle with a laser.

Claims15

Dependent claims

A method for detecting a test substance in a specimen, the method being

(A) a sandwich method comprising:
(1) bringing a specimen into contact with a magnetic particle on which a primary substance is immobilized and which has a particle diameter that does not cause interference with a wavelength for measurement with a laser, to form a primary substance-test substance complex of the primary substance and a test substance in the specimen;
(2) bringing the primary substance-test substance complex into contact with a secondary substance, to form a primary substance-test substance-secondary substance complex;
and
(3) detecting the primary substance-test substance-secondary substance complex on the magnetic particle with a laser;
(B) a sandwich method comprising:
(1) bringing a primary substance into contact with a test substance in a specimen and a secondary substance, to form a primary substance-test substance-secondary substance complex;
(2) binding the primary substance-test substance-secondary substance complex to a magnetic particle having a particle diameter that does not cause interference with a wavelength for measurement with a laser;
and
(3) detecting the primary substance-test substance-secondary substance complex on the magnetic particle with a laser;
(C) a competitive method comprising:
(1) bringing a specimen and a secondary substance into contact with a magnetic particle on which a primary substance is immobilized and which has a particle diameter that does not cause interference with a wavelength for measurement with a laser, to form a primary substance-secondary substance complex of the primary substance and the secondary substance;
and
(2) detecting the primary substance-secondary substance complex on the magnetic particle with a laser;
or
(D) a competitive method comprising:
(1) bringing a primary substance into contact with a specimen and a secondary substance, to form a primary substance-secondary substance complex of the primary substance and the secondary substance;
(2) binding the primary substance-secondary substance complex to a magnetic particle having a particle diameter that does not cause interference with a wavelength for measurement with a laser;
and
(3) detecting the primary substance-secondary substance complex on the magnetic particle with a laser.

17 more elements

A magnetic particle having a particle diameter that does not cause interference with a wavelength for measurement with a laser, the magnetic particle being for use in a method for detecting a test substance in a specimen, the method being a sandwich method (A) or (B), or a competitive method (C) or (D), the method comprising steps described below:

(A) a sandwich method comprising:
(1) bringing a specimen into contact with the magnetic particle on which a primary substance is immobilized, to form a primary substance-test substance complex of the primary substance and a test substance in the specimen;
(2) bringing the primary substance-test substance complex into contact with a secondary substance, to form a primary substance-test substance-secondary substance complex;
and
(3) detecting the primary substance-test substance-secondary substance complex on the magnetic particle with a laser;
(B) a sandwich method comprising:
(1) bringing a primary substance into contact with a test substance in a specimen and a secondary substance, to form a primary substance-test substance-secondary substance complex;
(2) binding the primary substance-test substance-secondary substance complex to the magnetic particle;
and
(3) detecting the primary substance-test substance-secondary substance complex on the magnetic particle with a laser;
(C) a competitive method comprising:
(1) bringing a specimen and a secondary substance into contact with the magnetic particle on which a primary substance is immobilized, to form a primary substance-secondary substance complex of the primary substance and the secondary substance;
and
(2) detecting the primary substance-secondary substance complex on the magnetic particle with a laser;
or
(D) a competitive method comprising:
(1) bringing a primary substance into contact with a specimen and a secondary substance, to form a primary substance-secondary substance complex of the primary substance and the secondary substance;
(2) binding the primary substance-secondary substance complex to the magnetic particle;
and
(3) detecting the primary substance-secondary substance complex on the magnetic particle with a laser.

17 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/123,474Filed 24 Oct 2023
Priority: JP 2022-17018824 Oct 2022
Published: 25 Jun 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification9 codes

G01N 33/54326
The carrier being characterised by its particulate form: magnetic particles
G01N 21/39
Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry:…
G01N 33/532
Production of immunochemical test materials: production of labelled immunochemicals
G01N 33/543
Immunoassay; Biospecific binding assay; Materials therefor: with an insoluble carrier for immobilising immunochemicals