US 2026/0177520 A1 · ENZYME-BASED ELECTROCHEMICAL INLINE SENSOR WITH MEASURABLE SERVICE LIFE

Abstract

An electrochemical enzyme-based inline sensor for detecting a concentration of one or more analytes in a measuring medium, comprises at least one first electrode configured as a working electrode; at least one second electrode configured as a counter electrode, wherein the at least one first electrode, at which an enzyme-based redox reaction takes place, is configured to detect and transmit generated electrical charges from starting operation of the inline sensor and to generate a charge analyte-dependent current flow resulting from the redox reaction; and an evaluation device. The evaluation device is embodied to determine the concentration of the one or more analytes from the generated charges; and determine a measurand from the generated charges, wherein the measurand is used to determine an enzyme activity at the at least one first electrode, wherein the inline sensor is embodied to be installed in a process vessel and to be subsequently heat-sterilized.

Claims20

Dependent claims

An electrochemical enzyme-based inline sensor for detecting a concentration of one or more analytes in a measuring medium, comprising:

at least one first electrode configured as a working electrode;
at least one second electrode configured as a counter electrode, wherein
the at least one first electrode, at which an enzyme-based redox reaction takes place, is configured to detect and transmit generated electrical charges from starting operation of the inline sensor and to generate a charge analyte-dependent current flow resulting from the redox reaction;
and
an evaluation device embodied to:
determine the concentration of the one or more analytes from the generated charges;
and
determine a measurand from the generated charges, wherein the measurand is used to determine an enzyme activity at the at least one first electrode, wherein the inline sensor is embodied to be installed in a process vessel and to be subsequently heat-sterilized.

5 more elements

A method for continuously determining a total enzyme activity of an electrochemical enzyme-based inline sensor comprising:

providing a redox enzyme-based electrochemical inline sensor for process measurement technology or for bioprocess measurement technology to continuously monitor a state of an enzyme, comprising:
at least one sensor element comprising an amount of an immobilized redox enzyme, wherein the at least one sensor element is electrically coupled to a first electrode and/or is connected thereto, wherein the first electrode is configured as a working electrode, and
a counter electrode;
determining time-dependent charges received at a measuring region in a form of a current flow;
evaluating the current flow with an evaluation device to determine a concentration of an analyte in a liquid measuring medium;
and
determining the total enzyme activity of the inline sensor via a measurand determined from the current flow, wherein the inline sensor is embodied to be installed in a process vessel and to be subsequently heat-sterilized.

4 more elements

Dependencies are inferred from the claim text.

Enzyme-based electrochemical inline sensor with measurable service life

Life of this patent

Enzyme-based electrochemical inline sensor with measurable service life

Life of this patent

Abstract

Claims20