Methods and compositions for enriching circulating cell-free nucleic acids in a biological sample
AssigneesCentre for Novostics, Tencent Healthcare (Shenzhen) Co., Ltd.
InventorsYuk-Ming Dennis Lo, Kwan Chee Chan, Peiyong Jiang, Pak Hang Peter Cheung, Man Tat Alexander Ng, Wei Liu, Hou Tim Lai, Biaobin Jiang
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods and compositions for improving the quantity and quality of cfDNAs in a bodily fluid of a subject by inhibiting the nuclease activity in the subject. The methods and compositions disclosed herein are particularly useful for subjects who have been identified that liquid biopsies are needed.
Claims42
A method of increasing a cell-free DNA (cfDNA) level in a bodily fluid of a subject, the method comprising:
- administering to the subject a therapeutically effective amount of a nuclease inhibitor, wherein the subject has been identified for a liquid biopsy to be assayed for cfDNA, and wherein the nuclease inhibitor binds to a nuclease and inhibits cleavage of the cfDNA in the bodily fluid, thereby increasing the cfDNA level in the subject.
A method of inhibiting activity of a nuclease in a subject, the method comprising:
- administering to the subject a therapeutically effective amount of a nuclease inhibitor that binds to the nuclease, thereby inhibiting the nuclease activity in the subject, wherein the subject has been identified for a liquid biopsy to be assayed for cfDNA.
42-75. (canceled)
Dependencies are inferred from the claim text.