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AssigneeNanjing Agricultural University
InventorsFang HUANG, Deyue YU, Zhongyi YANG, Dezhou HU, Guizhen KAN, Fei WU, Xuan CUI
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Use of a soybean protein phosphatase family gene GmPP2C11 is provided. A soybean gene GmPP2C11 encoding a GmPP2C11 protein has the nucleotide sequence of SEQ ID NO: 1. A PSCM-GmPP2C11 knockout vector is genetically transformed into soybean, and it is found that a resulting gene-edited material shows significantly higher hundred-seed weight and yield per plant than those of a receptor Jack. This indicates that the soybean gene GmPP2C11 encoding the soybean GmPP2C11 protein may increase the seed weight and yield of a plant through genetic engineering, thereby providing technical services for increasing the yield of crops, especially soybean.
A method for regulating a yield and/or a height of a plant, comprising subjecting the plant to genetic engineering with a soybean gene GmPP2C11 encoding a GmPP2C11 protein;
A method for improving a plant height, a hundred-seed weight, and a yield per plant of soybean by genetic engineering, comprising transforming to the soybean an sgRNA knockout vector comprising a soybean gene GmPP2C11 encoding a GmPP2C11 protein;
An sgRNA knockout vector for a soybean gene GmPP2C11 encoding a GmPP2C11 protein, wherein the sgRNA knockout vector knocks out the soybean GmPP2C11 gene.
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