Manipulation of dominant male sterility
AssigneePioneer Hi-Bred International, Inc.
InventorAndrew Mark Cigan
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Abstract
Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid.
Claims22
-145. (canceled)
A wheat promoter comprising:
- (a) a polynucleotide sequence of SEQ ID NO:
- 14 or SEQ ID NO:6, and wherein the promoter initiates transcription in a plant cell;
- or
- (b) a polynucleotide which is complementary to the full length polynucleotide of (a);
- wherein the polynucleotide of (a) or (b) is operably linked to a heterologous polynucleotide of interest.
A method for expressing a polynucleotide in a plant or a plant cell, said method comprising introducing into the plant or the plant cell a promoter operably linked to a polynucleotide of interest, wherein said promoter is heterologous with respect to the plant or plant cell, and wherein said promoter comprises:
- (a) a polynucleotide sequence of SEQ ID NO:
- 14 or SEQ ID NO:6, and wherein the promoter initiates transcription in a plant cell;
- or
- (b) a polynucleotide which is complementary to the full length polynucleotide of (a).
A method for expressing a polynucleotide preferentially in male reproductive tissues of a plant, said method comprising introducing into a plant cell an expression cassette and regenerating a plant from said plant cell, said plant having stably incorporated into its genome the expression cassette, said expression cassette comprising a promoter operably linked to a heterologous polynucleotide of interest, wherein said promoter comprises:
- (a) a polynucleotide sequence of SEQ ID NO:
- 14 or SEQ ID NO:6, and wherein the promoter initiates transcription in a plant cell;
- or
- (b) a polynucleotide which is complementary to the full length polynucleotide of (a).
Dependencies are inferred from the claim text.