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US 2026/0176637 A1Published application
Compositions and methods for producing circular polyribonucleotides
AssigneeFlagship Pioneering Innovations VI, LLC
InventorsVadim DUDKIN, Ki Young PAEK
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Abstract
The present disclosure relates, generally, to compositions and methods for producing, purifying, and using circular RNA.
Claims54
A linear polyribonucleotide having the formula 5′-(A)-(B)-(C)-(D)-(E)-(F)-(G)-3′, wherein:
- (A) comprises a 3′ half of Group I catalytic intron fragment from a T4 phage nrdB gene or nrdD gene;
- (B) comprises a 3′ splice site;
- (C) comprises a 3′ exon fragment;
- (D) comprises a polyribonucleotide cargo;
- (E) comprises a 5′ exon fragment;
- (F) comprises a 5′ splice site;
- and
- (G) comprises a 5′ half of Group I catalytic intron fragment from a T4 phage nrdB gene or nrdD gene;
- and wherein (A), (B), or (C) comprises a first annealing region comprising from 4 to 50 ribonucleotides and (E), (F), or (G) comprises a second annealing region comprising from 4 to 50 ribonucleotides,
- wherein the first annealing region and the second annealing region have from 80% to 100% complementarity, or
- the first annealing region and the second annealing region comprises from zero to 10 mismatched base pairs.
8 more elements
Dependencies are inferred from the claim text.