Compositions and methods for modifying a target nucleic acid
AssigneeThe Regents of the University of California
InventorsAlexander Marson, Theodore Lee Roth, Daniel Goodman, David-Huy Nhu Nguyen, Francis C. Szoka, JR.
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Abstract
The disclosure provides compositions and methods for modifying a target nucleic acid. In some embodiments, a composition can include a targetable nuclease, a DNA-binding protein, and a donor template comprising a homology directed repair (HDR) template and one or more DNA-binding protein target sequences. In some embodiments, a composition can include a Cas protein, one or more single guide RNAs (sgRNAs), and an anionic polymer.
Claims20
A composition for modifying a target nucleic acid, comprising:
- a. a targetable nuclease;
- and
- b. a donor template comprising a homology directed repair (HDR) template, one or more protospacer adjacent motifs (PAMs), and one or more DNA-binding protein target sequences.
A primary cell comprising a composition comprising:
- a. a targetable nuclease;
- and
- b. a donor template comprising a homology directed repair (HDR) template, one or more protospacer adjacent motifs (PAMs), and one or more DNA-binding protein target sequences.
A method for modifying a target nucleic acid in a cell, comprising introducing into the cell a composition comprising:
- a. a targetable nuclease;
- and
- b. a donor template comprising a homology directed repair (HDR) template, one or more protospacer adjacent motifs (PAMs), and one or more DNA-binding protein target sequences,
- wherein the HDR template is integrated into the target nucleic acid.
Dependencies are inferred from the claim text.