Variants of poly(a) polymerase and uses thereof
AssigneeDNA Script
InventorsTillmann HEINISCH, Elinor MORRIS, Morgane MELLARE, Guillaume VILLAIN, Clara MENDES, Elise CHAMPION, Mikhael SOSKINE
Life of this patent
Estimating the term…
Abstract
The present invention relates to poly(A) polymerase variants having several amino acid modifications which confer increased stability, increased activity and increased purity of the polynucleotides obtained therefrom during template-free polynucleotide synthesis. The present invention further relates to use of the variant enzymes for synthesising polynucleotides, methods of synthesising polynucleotides comprising the variant enzymes, and kits comprising the variant enzyme.
Claims15
A poly(A) polymerase variant comprising an amino acid sequence having at least 70% identity to SEQ ID NO:1 or a functional fragment thereof, and comprising mutations at residues V199, V240, and M318 of SEQ ID NO:1 or at functionally equivalent residues thereto.
Dependencies are inferred from the claim text.