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US 2026/0176334 A1Published application
SNAP-25 Antibodies and Methods of Using the Same
AssigneeGalderma Holding SA
InventorsRobert FREDRIKSSON, Lina GUDMUNDS, Emilia LEKHOLM, Hassani IMANE, Alexandra LENGQUIST, Karin NORDENANKAR, Elena MUSCOLINO, Julia SVENSSON
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Abstract
Antibodies that bind to SNAP-25 are provided. Botulinum toxin potency screening assays that utilize anti-SNAP-25 antibodies are also provided.
Claims20
An antibody fragment that binds to Synaptosomal-Associated Protein, 25 kDa (SNAP-25) and comprises a heavy chain region and optionally comprises a light chain region,
A method of quantifying SNAP-25 cleavage by botulinum neurotoxins (BoNTs), the method comprising:
- (a) incubating in a first series of containers a first population of cells that express SNAP-25 with a serial dilution of a first botulinum neurotoxin (BoNT) sample of known potency and incubating in a second series of containers a second population of cells that express SNAP-25 with a second serial dilution of a BoNT sample of unknown potency, wherein each incubation is for a defined period of time;
- (b) after the incubation, separating cleaved SNAP-25 from uncleaved SNAP-25 from each of the first population of cells and the second population of cells;
- (c) contacting cleaved and uncleaved SNAP-25 from each of the first population of cells and the second population of cells with an anti-Synaptosomal-Associated Protein, 25 kDa (SNAP-25) antibody or antigen-binding fragment thereof that comprises a heavy chain region and optionally comprises a light chain region,
- and quantifying the relative amount of cleaved and uncleaved SNAP-25 in each of the first population of cells and the second population of cells.
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Dependencies are inferred from the claim text.