N-linked glycan removal mutant antibodies and their use against influenza viruses

A synthetic antibody or fragment thereof comprising a variable heavy chain domain and a variable light chain domain, wherein the variable heavy chain domain of the synthetic antibody or fragment thereof comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:

• 23, and the variable light chain domain of the synthetic antibody or fragment thereof comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, or SEQ ID NO:
• 8.

A vector comprising at least one polynucleotide sequence encoding an antibody or fragment thereof, wherein the antibody or fragment thereof comprises a variable heavy chain domain and a variable light chain domain, wherein the variable heavy chain domain of the antibody or fragment thereof comprises SEQ ID NO:

• 23, and the variable light chain domain of the antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, and SEQ ID NO:
• 8.

A vector comprising at least one polynucleotide sequence encoding an antibody or fragment thereof, wherein the antibody or fragment thereof comprises a variable heavy chain domain and a variable light chain domain, wherein the variable heavy chain domain of the antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:

• 1, SEQ ID NO:
• 3, SEQ ID NO:
• 5, and SEQ ID NO:
• 7, and the variable light chain domain of the antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
• 17, SEQ ID NO:
• 18, SEQ ID NO:
• 19, SEQ ID NO:
• 20, SEQ ID NO:
• 21, and SEQ ID NO:
• 22.

A method of making an antibody or fragment thereof comprising:

• culturing a host cell under conditions suitable for an expression of a vector,
• wherein the vector comprises at least one polynucleotide sequence encoding the antibody or fragment thereof, wherein the antibody or fragment thereof comprises a variable heavy chain domain and a variable light chain domain, wherein the variable heavy chain domain of the antibody or fragment thereof comprises SEQ ID NO:
• 23, and the variable light chain domain of the antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
• 2, SEQ ID NO:
• 4, SEQ ID NO:
• 6, and SEQ ID NO:
• 8;
• and
• recovering the antibody or fragment thereof.

A method of preventing or treating an infection in a subject caused by an H7N9 influenza virus, the method comprising administering to the subject an effective amount of a pharmaceutical composition, wherein the pharmaceutical composition comprises:

• a synthetic antibody or fragment thereof comprising a variable heavy chain domain and a variable light chain domain, wherein the variable heavy chain domain of the synthetic antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
• 1, SEQ ID NO:
• 3, SEQ ID NO:
• 5, and SEQ ID NO:
• 7, and the variable light chain domain of the synthetic antibody or fragment thereof comprises an amino acid sequence selected from the group consisting of SEQ ID NO:
• 17, SEQ ID NO:
• 18, SEQ ID NO:
• 19, SEQ ID NO:
• 20, SEQ ID NO:
• 21, and SEQ ID NO:
• 22.