Frozen activated platelet compositions and collections, and methods of making and using the same

A collection of cryo-vessels, comprising

• a plurality of cryo-vessels, each cryo-vessel in the collection comprising 1 unit equivalent of frozen activated platelets comprising a population of platelet particles in a cryopreservation medium in a frozen state,
• wherein the population of platelet particles in the frozen activated platelets in each cryo-vessel have a set of biomolecule profiles indicative of more than 1 platelet donor, and
• wherein the frozen activated platelets in each cryo-vessel in the collection, exhibit the following properties, upon thawing:
• form a thawed activated platelet composition weighing between 24.50 and 30.50 grams when diluted with 25 ml of saline;
• have a capacity to generate thrombin in an in vitro thrombin generation assay, and
• have less than 10×106 CD61-positive microparticles/μl of the thawed activated platelet composition, and
• wherein the thawing comprises placing the cryo-vessel in a water bath set at a temperature of 37° C.+/−2° C. until the frozen activated platelets are thawed to form the thawed activated platelet composition.

A process for preparing a batch of a cryopreserved platelet composition comprising a population of platelet particles,

• comprising:
• a) forming a concentrated pooled platelet resuspension (CPR) by removing some plasma from pooled platelet units to achieve a weight or volume based on the number of platelet units (PU) to form the CPR;
• b) adding dimethyl sulfoxide (DMSO) to the CPR to obtain a CPR having DMSO
• c) distributing the CPR having DMSO among more than 1 cryo-vessel from a collection of cryo-vessels;
• and
• d) freezing the collection of cryo-vessels to prepare the batch of the cryopreserved platelet composition comprising the population of platelet particles,
• wherein the distributing is done to obtain 1 PU equivalent weight of the CPR having DMSO in each cryo-vessel, and
• wherein the freezing is initiated within 2 hours after adding the DMSO.