Brassica Plants Producing Elevated Levels of Polyunsaturated Fatty Acids

A Brassica plant or a part thereof comprising:

• (i) one or more T-DNAs heritably integrated into its genome, the T-DNAs comprising one or more expression cassettes having nucleotide sequences encoding one or more d12DES, one or more d6Elo, one or more d6Des, one or more d5Des, one or more d5Elo, one or more d4Des, one or more o3Des;
• or a combination thereof;
• and
• (ii) all or part of at least one genomic sequence of a B. napus parent genome that confers a higher polyunsaturated fatty acid content, wherein said genome sequence is selected from the group consisting of:
• a) the genomic sequence on chromosome N1 between nucleotide positions 8,879,780 and 11,922,690;
• b) the genomic sequence on chromosome N1 between nucleotide positions 22,823,086 and 24,045,492;
• and
• c) the genomic sequence on chromosome N6 between nucleotide positions 19,156,645 and 20,846,412;
• wherein seeds of said Brassica plant have a greater amount of one or more polyunsaturated fatty acids selected from the group consisting of EPA, DPA, and DHA than seeds of a control Brassica plant lacking (i) a (ii).

A method of producing a Brassica plant or a part thereof, said method comprising crossing a first Brassica parent plant producing one or more of DPA, DHA and/or EPA in its seeds with a second Brassica parent plant to produce progeny plants, wherein one or both of said first and said second Brassica parent plants comprises all or part of at least one genomic sequence of a B. napus parent genome that confers a higher polyunsaturated fatty acid content,

• wherein said genome sequence is selected from the group consisting of:
• a) the genomic sequence on chromosome N1 between nucleotide positions 8,879,780 and 11,922,690;
• b) the genomic sequence on chromosome N1 between nucleotide positions 22,823,086 and 24,045,492;
• and
• c) the genomic sequence on chromosome N6 between nucleotide positions 19,156,645 and 20,846,412;
• and
• wherein seeds of said Brassica plant have a greater amount of one or more polyunsaturated fatty acids selected from the group consisting of EPA, DPA, and DHA than seeds of said first parental Brassica plant and/or the second parental Brassica plant.