Compositions and methods for the detection and analysis of herpes simplex virus 1 (hsv-1), herpes simplex virus 2 (hsv-2) and varicella zoster virus (vzv)
OwnerJeffrey D. Wuitschick, Rupinder Kular +3inventors
InventorsJeffrey D. Wuitschick, Rupinder Kular, Tomasz Krupinski, Heather C. Alexander, Qingbei Zhang
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Abstract
Provided herein are compositions and methods useful for the detection of Herpes Simplex Virus 1 (HSV-1), Herpes Simplex Virus 2 (HSV-2) and Varicella Zoster Virus (VZV). In particular, provided herein are compositions, methods, systems, kits, reagents, and reaction mixtures involving such for nucleic acid amplification and detection procedures that detect HSV-1, HSV-2 and VZV in samples.
Claims35
A method of detecting Herpes Simplex Virus 1 (HSV-1), Herpes Simplex Virus 2 (HSV-2) and Varicella Zoster Virus (VZV) nucleic acid in a biological sample from a subject, comprising:
- a) extracting nucleic acid from said biological sample;
- b) contacting said nucleic acid from said biological sample from said subject with at least two nucleic acid primer pairs selected from the group consisting of SEQ ID NOS:
- 1 and 2, SEQ ID NOS:
- 4 and 5, SEQ ID NOS:
- 7 and 8, SEQ ID NOS:
- 10 and 11, SEQ ID NOS:
- 13 and 14, SEQ ID NOS:
- 16 and 17, and SEQ ID NOS:
- 19 and 20, and at least two probes selected from the group consisting of SEQ ID NOS:
- 3, 6, 9, 12, 15, 18 and 21;
- d) performing an amplification assay to amplify one or more HSV-1, HSV-2 and VZV nucleic acid targets;
- and
- e) detecting the presence of said HSV-1, HSV-2 and VZV nucleic acid targets in said sample.
A composition, comprising at least two nucleic acid primer pairs selected from the group consisting of SEQ ID NOS:
- 1 and 2, SEQ ID NOS:
- 4 and 5, SEQ ID NOS:
- 7 and 8, SEQ ID NOS:
- 10 and 11, SEQ ID NOS:
- 13 and 14, SEQ ID NOS:
- 16 and 17, and SEQ ID NOS:
- 19 and 20, and at least two probes selected from the group consisting of SEQ ID NOS:
- 3, 6, 9, 12, 15, 18 and 21.
A reaction mixture, comprising:
- a) at least two nucleic acid primer pairs selected from the group consisting of SEQ ID NOS:
- 1 and 2, SEQ ID NOS:
- 4 and 5, SEQ ID NOS:
- 7 and 8, SEQ ID NOS:
- 10 and 11, SEQ ID NOS:
- 13 and 14, SEQ ID NOS:
- 16 and 17, and SEQ ID NOS:
- 19 and 20, and at least two probes selected from the group consisting of SEQ ID NOS:
- 3, 6, 9, 12, 15, 18 and 21;
- and
- b) at least one reagent for performing a nucleic acid amplification reaction.
A system, comprising:
- a) at least two nucleic acid primer pairs selected from the group consisting of SEQ ID NOS:
- 1 and 2, SEQ ID NOS:
- 4 and 5, SEQ ID NOS:
- 7 and 8, SEQ ID NOS:
- 10 and 11, SEQ ID NOS:
- 13 and 14, SEQ ID NOS:
- 16 and 17, and SEQ ID NOS:
- 19 and 20, and at least two probes selected from the group consisting of SEQ ID NOS:
- 3, 6, 9, 12, 15, 18 and 21;
- and
- b) an automated sample handling instrument.
A kit, comprising:
- a) at least two nucleic acid primer pairs selected from the group consisting of SEQ ID NOS:
- 1 and 2, SEQ ID NOS:
- 4 and 5, SEQ ID NOS:
- 7 and 8, SEQ ID NOS:
- 10 and 11, SEQ ID NOS:
- 13 and 14, SEQ ID NOS:
- 16 and 17, and SEQ ID NOS:
- 19 and 20, and at least two probes selected from the group consisting of SEQ ID NOS:
- 3, 6, 9, 12, 15, 18 and 21;
- and
- b) at least one reagent for performing a nucleic acid amplification reaction.
Dependencies are inferred from the claim text.