Genotyping or sequencing platform with passivation layer
AssigneeIllumina, Inc.
InventorsHim Cheng Wong, Adithya Ramanitharapandian, Kenneth Chong, Maxwell Zimmerley, Leonid Krivitskiy, Sara Bakhshi
Life of this patent
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Abstract
An example of a genotyping or sequencing platform generally includes a negatively-charged substrate including a plurality of depressions defined therein that are separated by interstitial regions. The genotyping or sequencing platform further includes an exposed passivation layer positioned over an entirety of a surface of the negatively-charged substrate, wherein the exposed passivation layer at least partially fills each depression in the plurality of depressions and at least partially overlies each interstitial region across the entirety of the surface.
Claims21
A genotyping or sequencing platform, comprising:
- a negatively-charged substrate including a plurality of depressions defined therein that are separated by interstitial regions;
- and
- an exposed passivation layer positioned over an entirety of a surface of the negatively-charged substrate, wherein the exposed passivation layer aligns each depression in the plurality of depressions and at least partially overlies each interstitial region across the entirety of the surface.
A method of preparing a genotyping or sequencing platform surface, comprising:
- applying a passivation material over an entirety of a negatively-charged substrate that includes a plurality of depressions separated by interstitial regions defined therein, whereby the passivation material at least partially fills each depression in the plurality of depressions and at least partially overlies each of the interstitial regions, thereby forming an exposed passivation layer.
A genotyping kit, comprising:
- a genotyping platform, including:
- a negatively-charged substrate including a plurality of depressions defined therein separated by interstitial regions;
- and
- an exposed passivation layer positioned over an entirety of a surface of the negatively-charged substrate, wherein the exposed passivation layer at least partially fills each depression in the plurality of depressions and at least partially overlies each interstitial region across the entirety of the surface;
- a functionalized particle fluid, including:
- a liquid carrier;
- and
- a plurality of functionalized particles dispersed throughout the liquid carrier, each of the plurality of functionalized particles including:
- a particle core;
- and
- a plurality of a respective genotyping probe attached to the particle core.
A sequencing kit, comprising:
- a sequencing platform, including:
- a negatively-charged substrate including a plurality of depressions defined therein separated by interstitial regions;
- and
- an exposed passivation layer positioned over an entirety of a surface of the negatively-charged substrate, wherein the exposed passivation layer at least partially fills each depression in the plurality of depressions and at least partially overlies each interstitial region across the entirety of the surface;
- a functionalized particle fluid, including:
- a liquid carrier;
- and
- a plurality of functionalized particles dispersed throughout the liquid carrier, each of the plurality of functionalized particles including:
- a particle core;
- and
- a set of amplification primers attached to the particle core.
Dependencies are inferred from the claim text.