Excision of recombinant dna from the genome of plant cells

A method for transient expression of a gene of interest in a somatic plant cell, the method comprising the steps of

• a. introducing into the genome of said somatic cell one or more recombinant genetic elements each comprising
• i. a first excision recognition site,
• ii. a second excision recognition site, and between said first and second excision recognition site
• iii. a polynucleotide encoding excision components capable of excising the recombinant DNA located between said first and said second excision recognition site under control of a ntm19 promoter, and said gene of interest, and,
• b. expressing said excision components capable of excising the recombinant DNA located between said first and said second excision recognition site, wherein said excision components recognize said excision sites and excise said recombinant DNA located between said first and said second excision recognition site.

A method for improved regeneration of a transgenic plant from a somatic plant cell, the method comprising the steps of

• a. introducing into the genome of said somatic cell one or more recombinant genetic elements each comprising
• i. a first excision recognition site, and
• ii. a second excision recognition site, and between said first and second excision recognition site
• iii. a polynucleotide encoding excision components capable of excising the recombinant DNA located between said first and second excision recognition site under control of a ntm19 promoter and at least one morphogene functionally linked to a promoter, and
• b. expressing said excision components capable of excising the recombinant DNA located between said first and second excision recognition site, wherein said excision components recognize and excise said recombinant DNA located between said first and second excision recognition site.

A method to produce a plant or shoot comprising an edit in a target sequence, said method comprising:

• a. introducing into the genome of a somatic cell one or more recombinant genetic elements each comprising
• i. a first excision recognition site,
• ii. a second excision recognition site, and between said first and said second excision recognition site
• iii. a polynucleotide encoding excision components capable of excising the recombinant DNA located between said first and said second excision recognition site under control of a ntm19 promoter, and sequences encoding genome editing components for editing said target sequence
• b. regenerate shoots from said somatic cell,
• c. select shoots comprising said edit in said target sequence and in which excision has taken place of the recombinant DNA located between said first and said second excision recognition site, and optionally
• d. grow plants from said shoots.

A method for removal of genome editing components shortly after introduction of the targeted genomic modifications, said method comprising:

• a. introducing into the genome of a somatic cell one or more recombinant genetic elements each comprising
• i. a first excision recognition site,
• ii. a second excision recognition site, and between said first and second excision recognition site
• iii. a polynucleotide encoding excision components capable of excising the recombinant DNA located between said first and said second excision recognition site under control of a ntm19 promoter, and sequences encoding genome editing components for editing a target sequence
• b. regenerate shoots from said somatic cell, and
• c. select shoots comprising said edit in said target sequence and in which excision has taken place of the recombinant DNA located between said first and said second excision recognition site.