Preparation method and application for bionic enzyme aerogel reactor

Abstract

A preparation method and an application for a bionic enzyme aerogel reactor are provided. The preparation method includes following steps: step 1, first preparing titanium dioxide nanofibers (TiNFs) through solvothermal treatment; step 2, freeze-casting and vacuum-drying an aqueous dispersion including the TiNFs, chitosan methacryloyl (CSMA) and a crosslinking agent, to obtain a bionic CSMA/titanium dioxide nanofibers (C/TiNFs) aerogel reactor with vertically aligned channels; step 3, polymerizing and modifying glycidyl methacrylate (GMA), sulfobetaine methacrylate (SBMA) monomer and N,N′-methylenebisacrylamide (MBA) crosslinker on a skeleton of the bionic enzyme aerogel reactor; and step 4, covalently immobilizing tannase in the bionic enzyme aerogel reactor under mild conditions, thereby obtaining a bionic tannase-poly(GMA-SBMA-CSMA)/titanium dioxide nanofibers (pGSC/TiNFs) enzyme reactor.

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