Compositions and methods for the production of allulose
AssigneeGreenLight Biosciences, Inc.
InventorsMatthew Eduardo Moura, Andrew Simon, Shreya Sawant, Mark Welch, Sridhar Govindarajan
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Abstract
Provided herein, in some embodiments, are allulose 6-phosphate phosphatases having improved properties (e.g., increased thermostability) and methods of using same for converting allulose-6-phosphate to allulose.
Claims46
A mutant allulose 6-phosphate phosphatase (A6PP) comprising an amino acid sequence at least 85% identical to SEQ ID NO:
- 1 and further comprising one or more amino acid mutations at positions selected from the group consisting of:
- amino acid positions 41, 59, 89, 124, 140, and 142 of SEQ ID NO:
- 1.
A mutant allulose 6-phosphate phosphatase (A6PP) comprising the amino acid sequence of SEQ ID NO:
- 2.
A mutant allulose 6-phosphate phosphatase (A6PP) comprising an amino acid sequence at least 85% identical to SEQ ID NO:
- 1 and further comprising one or more amino acid mutations at positions selected from the group consisting of:
- amino acid positions 38, 41, 124, 140, 142 and 206 of SEQ ID NO:
- 1.
A mutant allulose 6-phosphate phosphatase (A6PP) comprising an amino acid sequence of any one of SEQ ID NOs:
- 2-26.
The mutant A6PP of any preceding claim, wherein the mutant A6PP has a half-life of at least about two hours at about 60° C.
The mutant A6PP of any preceding claim, wherein the mutant A6PP has a longer half-life than an A6PP having the amino acid sequence of SEQ ID NO:
- 1.
The mutant A6PP of any preceding claim, wherein the mutant A6PP is more selective for A6P relative to fructose 6-phosphate and/or glucose 6-phosphate than an A6PP having the amino acid sequence of SEQ ID NO:
- 1.
A nucleic acid comprising a nucleotide sequence at least 85% identical to any one of SEQ ID NO:
- 47-69.
Dependencies are inferred from the claim text.