Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment

A method of culturing an attachment-dependent cell within a microfluidic environment, comprising:

• introducing an attachment-dependent cell into a chamber of a microfluidic device, wherein the microfluidic device includes a base, a microfluidic circuit structure disposed on the base, and a cover which collectively define a microfluidic circuit,
• wherein said microfluidic circuit comprises:
• a flow region for containing a flow of a first fluidic medium, wherein one or more inner surfaces of the flow region comprises a first surface coating;
• and
• the chamber, wherein the chamber has a single opening to the flow region, and wherein one or more inner surface of the chamber includes a second surface coating, wherein the second surface coating promotes adhesion of the attachment-dependent cell;
• culturing the attachment-dependent cell in the chamber for a first period of time;
• disengaging the attachment-dependent cell from the coated inner surface of the chamber;
• and
• exporting the attachment-dependent cell from the chamber.