Chimeric protein and expression system

Abstract

There is described a novel chimeric protein which reduces oxidative stress in a host cell, which comprises a Bol3 polypeptide operably linked to a Lip5 polypeptide. The chimeric protein can include a linker between the Bol3 polypeptide and the Lip5 polypeptide. The linker can be a polyhistidine linker. Also described is a polynucleotide encoding the chimeric protein, a vector incorporating the polynucleotide, and a host cell transformed with the vector. A method of expressing a target polypeptide having at least one disulfide bond (for example at least three disulfide e bonds, for example a target polypeptide having an ICK) within a eukaryotic host cell, said method comprising transforming said host cell with a polynucleotide encoding the chimeric protein and culturing said host cell under conditions wherein said chimeric protein and said polypeptide are both expressed.

Dependencies are inferred from the claim text.