US 2026/0167680 A1 · NOVEL LIGHT-ACTIVATED CHANNEL PROTEIN VR3.0 AND USES THEREOF

Abstract

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

Claims20

Dependent claims

A light-activated channel protein comprising a channelrhodopsin comprising an amino acid sequence shown in SEQ ID NO.1 or a variant thereof, wherein the variant is selected from any one of the following:

(1) a protein obtained by truncating 1-23 amino acids at the N-terminus of the channelrhodopsin;
(2) a protein obtained by truncating 90-189 amino acids at the C-terminus of the channelrhodopsin;
(3) a protein obtained by truncating 1-23 amino acids at the N-terminus and 90-189 amino acids at the C-terminus of the channelrhodopsin;
or
(4) a protein having more than 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity with the protein described in (1), (2), or (3).

2 more elements

Dependencies are inferred from the claim text.

Novel light-activated channel protein vr3.0 and uses thereof

Life of this patent

Novel light-activated channel protein vr3.0 and uses thereof

Life of this patent

Abstract

Claims20