System and method for fluidics cartridge comprising semiconductor array for biomarker detection
OwnerBryan Joseph Riceinventor
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Abstract
The present invention is directed to a system and method for a research instrument device. A method of purifying extracellular vesicles (“EVs”) is disclosed wherein a biological sample including EVs is obtained and applied to a fluidic cell in an array of fluidic cells of a multistage dielectrophoretic (“DEP”) filter system. The DEP filter system comprises a semiconductor array comprising a plurality of electrodes. The DEP filter system is disposed within a fluidics system; wherein the fluidics system is configured to move at least one fluid across the semiconductor array of the DEP filter system.
Claims20
A system for a fluidics cartridge comprising a semiconductor array for biomarker detection, the system comprising:
- a cartridge body comprising:
- a plurality of pipette tips;
- a pierceable well cover;
- a cartridge base comprising:
- a plurality of reagent storage wells,
- a tip storage area,
- a sample inlet well,
- an elution outlet well,
- mating features for a flow cell,
- a waste reservoir, and
- a pogo pin access;
- a flow cell comprising:
- a flow cell base comprising:
- a plurality of separate parts,
- an inlet docking port,
- an outlet docking port,
- a pogo pin access,
- mating features for a pressure-sensitive adhesive (PSA) spacer,
- mating features for said flow cell base,
- a dielectrophoresis (DEP) chip,
- a PSA spacer,
- a top sealing film, and
- a bottom sealing film.
A method for biomarker detection using a fluidics cartridge comprising a semiconductor array, the method comprising:
- introducing a sample fluid into the cartridge;
- priming the cartridge with a prime buffer to prepare it for fluid flow;
- pumping and recirculating at least one fluid within a flow cell comprising the semiconductor array;
- washing the cartridge with a wash buffer;
- washing the cartridge with an elution buffer under capture conditions;
- and
- eluting the cartridge with an elution buffer under release conditions.
A system for a fluidics cartridge comprising a semiconductor array configured to receive and process a fluid for biomarker detection, the system comprising:
- a cartridge body comprising:
- a cartridge base comprising:
- a plurality of reagent storage wells,
- a tip storage area,
- a sample inlet well,
- an elution outlet well,
- a handle,
- mating features for a flow cell,
- a waste reservoir, and
- a pogo pin access,
- a plurality of pipette tips;
- and
- a pierceable well cover;
- a flow cell comprising:
- a plurality of heat stakes;
- and
- a flow cell base comprising:
- a plurality of separate parts,
- an inlet docking port,
- an outlet docking port,
- a pogo pin access,
- mating features for PSA spacer,
- mating features for said flow cell base,
- a dielectrophoresis chip comprising a semiconductor array configured from a plurality of electrodes, wherein each of said plurality of electrodes is composed of a layer of metal, layer of dielectric material, and a layer of substrate,
- a PSA spacer,
- a top sealing film, and
- a bottom sealing film.
Dependencies are inferred from the claim text.