System and method for semiconductor-based isolation of extracellular vesicles

A method for controlling a motor system configured to isolate extracellular vesicles, the method comprising:

• running a motor control algorithm via a GUI on a computer system in
• communication with a cartridge device, said motor control algorithm causing a firmware having an application framework to:
• process and record motor movement speed and step number via a firmware for at least one motor;
• transmit, via said firmware, one-time setup parameters to a plurality of peripherals;
• establish real-time task expiry time for each motor based on a designated speed;
• create, via said firmware, at least one step counter for at least one motor and set a state of said at least one motor to running as part of a real-time event-handling protocol;
• check for service updates of said at least one motor;
• and
• running a temperature control timing algorithm to provide constant temperature in
• a fluid integrated circuit (IC) chip via a PID controller, said temperature control timing algorithm causing said firmware to:
• receive a temperature set point request;
• process and record said temperature set point request;
• send one-time setup parameters to various peripherals;
• set up real-time task expiry time for each TEC based on indicated settings;
• set up a recurring timer to send a message to process TEC controller;
• check message queue via a main thread;
• and
• upon schedule, service a TEC controller by running a current measured temperature through a dedicated PID controller routine to be sent again at a same interval defined by said recurring timer.

A system for semiconductor-based isolation of extracellular vesicles, the system comprising:

• a cartridge device comprising:
• an x-axis system comprising a plurality of subsystems including a pump and pressure subsystem;
• a z-axis system comprising a pipette tip interface, a tip eject, and tip present sensor subsystem;
• at least one lift axis system comprising a plurality of heatsinks, a plurality of pogo pin connections, and a pinch valve subsystem;
• a plurality of cartridge bays, each of said cartridge bay comprising a plurality of partitions, a plurality of cartridge receivers, and a plurality of LED indicators;
• a door system;
• at least one auxiliary device;
• a plurality of stepper motors;
• a plurality of assembly components comprising:
• a pipette gantry head,
• a pogo pin lifter assembly,
• a Peltier lifter,
• a pinch valve filter,
• a cartridge receiver,
• a back plate, and
• at least one side plate;
• firmware configured to process and record information and create step counters for at least one of said plurality of stepper motors;
• a PID controller in communication with said firmware, wherein said PID controller provides pulse-width modulation of a TEC device to maintain temperature control;
• and
• a computer system in communication with said cartridge device, said computer system configured to house a GUI software application configured to allow a user to choose a method to drive hardware interactions via text-based recipe files.