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US 2026/0166546 A1Published application
Device and methods for isolating microorganisms from biological samples for diagnostic analysis
AssigneeSiemens Healthcare Diagnostics Inc.
InventorDavid Ledden
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Abstract
A method of isolating microorganisms present in a biological sample for diagnostic analysis, such as a diagnostic assay for sepsis. The method involves the use of filtration and acoustophoresis to substantially lyse any blood cells present in the biological sample and isolate any microorganisms present in the biological sample from the lysed cellular debris. Also disclosed are microfluidic devices, kits, and systems that can be utilized in the method.
Claims20
A method of isolating microorganisms present in a biological sample for diagnostic analysis, the method comprising:
- (1) diluting the biological sample in a buffer to provide a mixture;
- (2) pre-filtering the mixture to remove debris by passing the mixture through a first filter membrane, thereby providing a pre-filtered mixture;
- (3) exposing the pre-filtered mixture to acoustophoresis at a frequency sufficient to substantially lyse blood cells present in the mixture;
- and
- (4) filtering the acoustophoresis-treated mixture to remove debris resulting from the blood cell lysis by passing the acoustophoresis-treated mixture through a second filter membrane, whereby any microorganisms present in the biological sample are isolated on the second filter membrane.
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